N. meningitidis Serogroups
Dia-MenSerogroup-020
Neisseria meningitidis (gram-negative bacteria) leads to meningitis, and is thus responsible for considerable mortality throughout the world. Most disease-causing Neisseira meningitidis strains belong to serogroups B and C in Europe, North & South America and Australia.
VALIDATION |
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| Researched pathogens | • Neisseria meningitidis (all strains): FAM dye, emission: 520 nm • Serogroup A: FAM dye, emission: 520 nm • Serogroup B: FAM dye, emission: 520 nm • Serogroup C: FAM dye, emission: 520 nm • Serogroup W135: FAM dye, emission: 520 nm • Serogroup Y: FAM dye, emission: 520 nm |
| Target genes | • CtrA (Neisseria meningitidis) • sacB (Serogroup A) • siaD (Serogroup B) • siaD (Serogroup C) • synG (Serogroup W135) • synF (Serogroup Y) |
| Number of wells by test | 6 (Neisseria meningitidis and serogroup A, B, C, W135 and Y) |
| Samples used | • Human cerebrospinal fluid • Whole blood |
| Validated extraction methods | • Nuclisens easyMAG® System (Biomerieux) • QIAamp® DNA Mini Kit (50) (Blood and Body Fluid Spin Protocol) (Qiagen) • MagNa pure LC system (Roche) (Ongoing) |
| Real-time PCR systems | • ABI (7000/7300/7500/7900) • Roche LightCycler® (2.0/480) • Biorad (iCycler/IQ5/CFX96/Dx) • Qiagen Rotor-Gene (6000) • Stratagene (MX3000P/3005P) • Cepheid (SmartCycler II) |
| PCR volumes | 50 µl, 25 µl, 20 µl (depending of the real-time PCR system used) |
| PCR reactions / kit | • 20 reactions (50 µl PCR, final volume) • 40 reactions (25 µl PCR, final volume) • 50 reactions (20 µl PCR, final volume) |
| In combination with | Universal inhibition control (DIA-UIC-050): • DIA-UIC(YD)-050: yellow dye, emission: 549 nm • DIA-UIC(DR)-050: orange dye, emission: 576 nm • DIA-UIC(TR)-050: texas red dye, emission: 603 nm • DIA-UIC(Cy5)-050: Cy5 dye, emission: 662 nm DNA extraction & PCR inhibition control (DIA-EIC/DNA)-050: |
