Shigella

Dia-Shigella-050

Shigella is a genus of Gram-negative, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella.

Shigella species are classified by four serogroups:

• Serogroup A: S. dysenteriae (12 serotypes)
• Serogroup B: S. flexneri (6 serotypes)
• Serogroup C: S. boydii (23 serotypes)
• Serogroup D: S. sonnei (1 serotype)

Shigella causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7 (1).

Both Shiga toxin and verotoxin are associated with causing hemolytic uremic syndrome.

(1) Hale TL, Keusch GT (1996). Shigella. in: Baron’s Medical Microbiology (Baron S et al., eds.) (4th ed.). Univ of Texas Medical Branch

Patogeni ricercati	Shigella: FAM dye, emissione 520 nm
Geni Target	ipaH (Shigella)
Nr well/test	1 (enterotossina A/enterotossina B)
Campioni	feci
. Metodiche validate di estrazione	Nuclisens easyMAG® System (Biomerieux) QIAamp DNA Stool mini kit (Qiagen) MagNa pure LC system (Roche) (Ongoing) QiaSymphony (Qiagen)
. Termociclatori Real Time PCR	ABI (7000/7300/7500/7900) Roche LightCycler® (480/2.0 on request) Biorad (iCycler/IQ5/CFX96) Qiagen Rotor-Gene (6000) Stratagene (MX3000P/3005P) Cepheid (SmartCycler II)
Volume PCR	50 µl, 25 µl, 20 µl (dipende dallo strumento utilizzato)
. Reazioni PCR/kit	50/50 µl 100/25 µl 120/20µl
. In associazione con	Universal inhibition control (DIA-UIC-050): DIA-UIC(DR)-050: orange dye, emissione 576 nm DIA-UIC(TR)-050: texas red dye, emissione 603 nm DIA-UIC(Cy5)-050: Cy5 dye, emissione 662 nm DNA extraction & PCR inhibition control (DIA-EIC/DNA)-050: DIA-EIC/DNA(DR)-050: orange dye, emissione 576 nm DIA-EIC/DNA(TR)-050: texas red dye, emissione 603 nm DIA-EIC/DNA(Cy5)-050: Cy5 dye, emissione 662 nm